The formation of fowl plague virus antigens in infected cells, as studied with fluorescent antibodies

The multiplication of fowl plague virus was followed in tissue culture using fluorescent antibodies against its two antigenic components. With this method the g-antigen was first detected 3 hours after infection and the hemagglutinin antigen after 4 hours. The g-antigen was situated in the region of the nucleus whereas the hemagglutinin antigen was distributed throughout the cell and is located in a higher concentration in a juxtanuclear locus. Later the g-antigen is also found in the cytoplasm; the hemagglutinin antigen accumulates in the peripheral region of the cell and also may be demonstrated in fine filaments which protrude from the cell margin.