Differentiation of Escherichia, Enterobacter, and Klebsiella by Thin-Layer Chromatography
Summary A thin layer chromatographic method has been developed for differentiation of the genera Escherichia, Enterobacter, and Klebsiella based on the amounts of succinic, fumaric, and pyruvic acids formed during fermentative growth. The method employs only a single colony of bacterial cells and ca...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1973-11, Vol.144 (2), p.551-554 |
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| Sprache: | eng |
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| container_end_page | 554 |
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| container_issue | 2 |
| container_start_page | 551 |
| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 144 |
| creator | Altenbern, Robert A. |
| description | Summary
A thin layer chromatographic method has been developed for differentiation of the genera Escherichia, Enterobacter, and Klebsiella based on the amounts of succinic, fumaric, and pyruvic acids formed during fermentative growth. The method employs only a single colony of bacterial cells and can be completed in 4.5 hr. |
| doi_str_mv | 10.3181/00379727-144-37633 |
| format | Article |
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A thin layer chromatographic method has been developed for differentiation of the genera Escherichia, Enterobacter, and Klebsiella based on the amounts of succinic, fumaric, and pyruvic acids formed during fermentative growth. The method employs only a single colony of bacterial cells and can be completed in 4.5 hr.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-144-37633</identifier><identifier>PMID: 4583623</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Chromatography, Thin Layer ; Enterobacter - classification ; Enterobacteriaceae - isolation & purification ; Enterobacteriaceae - metabolism ; Escherichia - classification ; Escherichia coli - classification ; Fermentation ; Fumarates - analysis ; Fumarates - biosynthesis ; Klebsiella - classification ; Klebsiella pneumoniae - classification ; Pyruvates - analysis ; Pyruvates - biosynthesis ; Succinates - analysis ; Succinates - biosynthesis</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1973-11, Vol.144 (2), p.551-554</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-3391aac9ec4a1b9ccee08b378a8e9eaa37afcf0b0c3d0abcb9c63f95ef6d1efa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4583623$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Altenbern, Robert A.</creatorcontrib><title>Differentiation of Escherichia, Enterobacter, and Klebsiella by Thin-Layer Chromatography</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
A thin layer chromatographic method has been developed for differentiation of the genera Escherichia, Enterobacter, and Klebsiella based on the amounts of succinic, fumaric, and pyruvic acids formed during fermentative growth. The method employs only a single colony of bacterial cells and can be completed in 4.5 hr.</description><subject>Chromatography, Thin Layer</subject><subject>Enterobacter - classification</subject><subject>Enterobacteriaceae - isolation & purification</subject><subject>Enterobacteriaceae - metabolism</subject><subject>Escherichia - classification</subject><subject>Escherichia coli - classification</subject><subject>Fermentation</subject><subject>Fumarates - analysis</subject><subject>Fumarates - biosynthesis</subject><subject>Klebsiella - classification</subject><subject>Klebsiella pneumoniae - classification</subject><subject>Pyruvates - analysis</subject><subject>Pyruvates - biosynthesis</subject><subject>Succinates - analysis</subject><subject>Succinates - biosynthesis</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhi0EKqXwB5CQMjE11M7lc0SlfIhKLGVgsi7uuTFq42InQ_49CS2MTDfc8766exi7FvwORC5mnENWZFEWijgOIUsBTthYJJCEkBbFKRsPQDgQ5-zC-0_ORZJF6YiN4iSHNIIx-3gwWpOjujHYGFsHVgcLrypyRlUGp8GibsjZElU_pgHW6-B1S6U3tN1iUHbBqjJ1uMSOXDCvnN1hYzcO91V3yc40bj1dHeeEvT8uVvPncPn29DK_X4YKIG9CgEIgqoJUjKIslCLieQlZjjkVhAgZaqV5yRWsOZaqR1LQRUI6XQvSCBN2e-jdO_vVkm_kzng1nFeTbb3MIw79r3EPRgdQOeu9Iy33zuzQdVJwOfiUvz5l71P--OxDN8f2ttzR-i9yFNjvZ4e9xw3JT9u6un_2v8ZvpoaAWg</recordid><startdate>197311</startdate><enddate>197311</enddate><creator>Altenbern, Robert A.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197311</creationdate><title>Differentiation of Escherichia, Enterobacter, and Klebsiella by Thin-Layer Chromatography</title><author>Altenbern, Robert A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-3391aac9ec4a1b9ccee08b378a8e9eaa37afcf0b0c3d0abcb9c63f95ef6d1efa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Chromatography, Thin Layer</topic><topic>Enterobacter - classification</topic><topic>Enterobacteriaceae - isolation & purification</topic><topic>Enterobacteriaceae - metabolism</topic><topic>Escherichia - classification</topic><topic>Escherichia coli - classification</topic><topic>Fermentation</topic><topic>Fumarates - analysis</topic><topic>Fumarates - biosynthesis</topic><topic>Klebsiella - classification</topic><topic>Klebsiella pneumoniae - classification</topic><topic>Pyruvates - analysis</topic><topic>Pyruvates - biosynthesis</topic><topic>Succinates - analysis</topic><topic>Succinates - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Altenbern, Robert A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Altenbern, Robert A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentiation of Escherichia, Enterobacter, and Klebsiella by Thin-Layer Chromatography</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1973-11</date><risdate>1973</risdate><volume>144</volume><issue>2</issue><spage>551</spage><epage>554</epage><pages>551-554</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
A thin layer chromatographic method has been developed for differentiation of the genera Escherichia, Enterobacter, and Klebsiella based on the amounts of succinic, fumaric, and pyruvic acids formed during fermentative growth. The method employs only a single colony of bacterial cells and can be completed in 4.5 hr.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>4583623</pmid><doi>10.3181/00379727-144-37633</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1973-11, Vol.144 (2), p.551-554 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82033624 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Chromatography, Thin Layer Enterobacter - classification Enterobacteriaceae - isolation & purification Enterobacteriaceae - metabolism Escherichia - classification Escherichia coli - classification Fermentation Fumarates - analysis Fumarates - biosynthesis Klebsiella - classification Klebsiella pneumoniae - classification Pyruvates - analysis Pyruvates - biosynthesis Succinates - analysis Succinates - biosynthesis |
| title | Differentiation of Escherichia, Enterobacter, and Klebsiella by Thin-Layer Chromatography |
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