Micro method for determination of borohydride with NAD

Micro method for determination of borohydride with NAD

A spectrophotometric method for the determination of borohydride is described. It involves the reduction of NAD + to a number of isomeric forms of NADH by borohydride. In the standard assay procedure herewith presented, there is a direct proportionality between the absorption at 340 nm and the amoun...

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Veröffentlicht in:Analytical biochemistry 1973-08, Vol.54 (2), p.554-560
Hauptverfasser: Werner, D.A., Huang, Chin C., Aminoff, David
Format: Artikel
Sprache:eng
Schlagworte:
Borohydrides - analysis
Buffers
Hydrogen-Ion Concentration
Methods
NAD
Spectrophotometry, Ultraviolet
Temperature
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Zusammenfassung:A spectrophotometric method for the determination of borohydride is described. It involves the reduction of NAD + to a number of isomeric forms of NADH by borohydride. In the standard assay procedure herewith presented, there is a direct proportionality between the absorption at 340 nm and the amount of borohydride in solution over the range 10–100 nmoles, with an effective “molar extinction coefficient” of 12.2 × 10 3. The method is simple, rapid, and sensitive.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(73)90387-4