A method for the assay of chymotrypsin in crude biological materials

A method for the assay of chymotrypsin in crude biological materials

A method suitable for assaying chymotrypsin in crude biological materials such as feces has been described. The procedure employs a water soluble peptide, N-benzoyl- l-tyrosyl-para-aminobenzoate (Na), which is hydrolyzed by chymotrypsin ( K m = 2.9 × 10 −3 M) to yield the TCA-soluble diazotizable ar...

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Veröffentlicht in:Analytical biochemistry 1973-01, Vol.54 (1), p.199-204
Hauptverfasser: Imondi, A.R., Stradley, R.P., Butler, E.R., Wolgemuth, R.L.
Format: Artikel
Sprache:eng
Schlagworte:
Aminobenzoates
Animals
Azo Compounds
Benzoates
Chymotrypsin - analysis
Chymotrypsin - metabolism
Dipeptides
Feces - analysis
Hydrogen-Ion Concentration
Kinetics
Methods
Pancreatin - analysis
Solubility
Spectrophotometry
Swine
Tyrosine
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Beschreibung
Zusammenfassung:A method suitable for assaying chymotrypsin in crude biological materials such as feces has been described. The procedure employs a water soluble peptide, N-benzoyl- l-tyrosyl-para-aminobenzoate (Na), which is hydrolyzed by chymotrypsin ( K m = 2.9 × 10 −3 M) to yield the TCA-soluble diazotizable aromatic amine.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(73)90263-7