Rapid turnover of principal phospholipids in BHK-21 cells
When BHK-21 cells are pulse-labeled with (2- 3H)-glycerol for 1 hr, followed by chase periods of 0–22 hrs, rapid turnover of the newly synthesized major phospholipids is observed 2–6 hrs after termination of the pulse, indicating a half-life of 2.0–2.5 hrs. These lipids are also labeled to equilibri...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1973-06, Vol.52 (4), p.1252-1256 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 52 |
| creator | Gallaher, William R. Weinstein, David B. Blough, Herbert A. |
| description | When BHK-21 cells are pulse-labeled with (2-
3H)-glycerol for 1 hr, followed by chase periods of 0–22 hrs, rapid turnover of the newly synthesized major phospholipids is observed 2–6 hrs after termination of the pulse, indicating a half-life of 2.0–2.5 hrs. These lipids are also labeled to equilibrium rapidly, within 8 hrs, indicating that the bulk of these lipids participates in this rapid turnover. |
| doi_str_mv | 10.1016/0006-291X(73)90635-9 |
| format | Article |
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3H)-glycerol for 1 hr, followed by chase periods of 0–22 hrs, rapid turnover of the newly synthesized major phospholipids is observed 2–6 hrs after termination of the pulse, indicating a half-life of 2.0–2.5 hrs. These lipids are also labeled to equilibrium rapidly, within 8 hrs, indicating that the bulk of these lipids participates in this rapid turnover.</description><subject>Animals</subject><subject>Carbon Isotopes</subject><subject>Cell Line</subject><subject>Chromatography, Thin Layer</subject><subject>Cricetinae</subject><subject>Glycerol - metabolism</subject><subject>Half-Life</subject><subject>Isotope Labeling</subject><subject>Kidney - metabolism</subject><subject>Kinetics</subject><subject>Phospholipids - metabolism</subject><subject>Time Factors</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LxDAQxYMo67r6DRR6Ej1UJ02bPxdBF3XFBUEUvIU0STHSbWvSLvjtTd1ljx6GOcybN_N-CJ1iuMKA6TUA0DQT-OOCkUsBlBSp2ENTDALSDEO-j6Y7ySE6CuELAOOcigma5IxQgWGKxKvqnEn6wTft2vqkrZLOu0a7TtVJ99mGWLWLkpC4JrlbPEfrRNu6DsfooFJ1sCfbPkPvD_dv80W6fHl8mt8uU51npE9LCkzhjFOOqTK84LkypSgYUMOYLay2VFCIxUmuLDe6BJbxnNKSgzBVSWbofOPb-fZ7sKGXKxfGD1Rj2yFIjgVlRPAozDdC7dsQvK1kTLJS_kdikCMxOeKQIw7JiPwjJkVcO9v6D-XKmt3SFlGc32zmNoZcO-tl0M422hrnre6lad3_B34Bv_t42A</recordid><startdate>19730619</startdate><enddate>19730619</enddate><creator>Gallaher, William R.</creator><creator>Weinstein, David B.</creator><creator>Blough, Herbert A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19730619</creationdate><title>Rapid turnover of principal phospholipids in BHK-21 cells</title><author>Gallaher, William R. ; Weinstein, David B. ; Blough, Herbert A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-b607a1286816ad8584adb95706d77e5ece6960696834ae8dcb0728466b809dfb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Animals</topic><topic>Carbon Isotopes</topic><topic>Cell Line</topic><topic>Chromatography, Thin Layer</topic><topic>Cricetinae</topic><topic>Glycerol - metabolism</topic><topic>Half-Life</topic><topic>Isotope Labeling</topic><topic>Kidney - metabolism</topic><topic>Kinetics</topic><topic>Phospholipids - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gallaher, William R.</creatorcontrib><creatorcontrib>Weinstein, David B.</creatorcontrib><creatorcontrib>Blough, Herbert A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gallaher, William R.</au><au>Weinstein, David B.</au><au>Blough, Herbert A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid turnover of principal phospholipids in BHK-21 cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1973-06-19</date><risdate>1973</risdate><volume>52</volume><issue>4</issue><spage>1252</spage><epage>1256</epage><pages>1252-1256</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>When BHK-21 cells are pulse-labeled with (2-
3H)-glycerol for 1 hr, followed by chase periods of 0–22 hrs, rapid turnover of the newly synthesized major phospholipids is observed 2–6 hrs after termination of the pulse, indicating a half-life of 2.0–2.5 hrs. These lipids are also labeled to equilibrium rapidly, within 8 hrs, indicating that the bulk of these lipids participates in this rapid turnover.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4736910</pmid><doi>10.1016/0006-291X(73)90635-9</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biochemical and biophysical research communications, 1973-06, Vol.52 (4), p.1252-1256 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81967398 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Carbon Isotopes Cell Line Chromatography, Thin Layer Cricetinae Glycerol - metabolism Half-Life Isotope Labeling Kidney - metabolism Kinetics Phospholipids - metabolism Time Factors |
| title | Rapid turnover of principal phospholipids in BHK-21 cells |
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