Rapid turnover of principal phospholipids in BHK-21 cells

Rapid turnover of principal phospholipids in BHK-21 cells

When BHK-21 cells are pulse-labeled with (2- 3H)-glycerol for 1 hr, followed by chase periods of 0–22 hrs, rapid turnover of the newly synthesized major phospholipids is observed 2–6 hrs after termination of the pulse, indicating a half-life of 2.0–2.5 hrs. These lipids are also labeled to equilibri...

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Veröffentlicht in:Biochemical and biophysical research communications 1973-06, Vol.52 (4), p.1252-1256
Hauptverfasser: Gallaher, William R., Weinstein, David B., Blough, Herbert A.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Carbon Isotopes
Cell Line
Chromatography, Thin Layer
Cricetinae
Glycerol - metabolism
Half-Life
Isotope Labeling
Kidney - metabolism
Kinetics
Phospholipids - metabolism
Time Factors
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Zusammenfassung:When BHK-21 cells are pulse-labeled with (2- 3H)-glycerol for 1 hr, followed by chase periods of 0–22 hrs, rapid turnover of the newly synthesized major phospholipids is observed 2–6 hrs after termination of the pulse, indicating a half-life of 2.0–2.5 hrs. These lipids are also labeled to equilibrium rapidly, within 8 hrs, indicating that the bulk of these lipids participates in this rapid turnover.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(73)90635-9