Primary sites of virus multiplication following intramuscular inoculation of poliomyelitis virus in cynomolgus monkeys

The intramuscular inoculation of a virulent type 1 poliomyelitis virus was used to delineate primary sites of virus multiplication in cynomolgus monkeys. The virus disappeared insofar as it was not detected in tissues examined for a period of about 30 hours. Virus reappeared first in skeletal muscle at the site of inoculation. The evidence indicated multiplication of virus in cellular constituents in the muscle mass. Shortly thereafter virus appeared in collateral lymph nodes, and after a further brief interval in the blood stream. After onset of viremia, virus was widely distributed in neural and extraneural tissues.