Cultivation in Tissue-Culture of Cytopathogenic Agent from Bovine Mucosal Disease.
Summary Isolation and cultivation in tissue culture of a cytopathogenic agent recovered from tissues of cattle with mucosal disease from 2 separated herds is reported. A homologous tissue culture system was used with bovine embryonic kidney cells and newborn, antibody-free calf serum. The virus was...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1957-04, Vol.94 (4), p.795-797 |
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| container_issue | 4 |
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| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 94 |
| creator | Underdahl, N. R. Grace, O. D. Hoerlein, A. B. |
| description | Summary
Isolation and cultivation in tissue culture of a cytopathogenic agent recovered from tissues of cattle with mucosal disease from 2 separated herds is reported. A homologous tissue culture system was used with bovine embryonic kidney cells and newborn, antibody-free calf serum. The virus was inactivated by temperatures above 50°C for 15 minutes, would pass an 03 Selas filter and had an 11th passage titer of 10-5. In sheep, the virus stimulated formation of neutralizing antibodies. A preliminary neutralization study indicated that antibodies are common in animals from herds having no previous history of mucosal disease. |
| doi_str_mv | 10.3181/00379727-94-23091 |
| format | Article |
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Isolation and cultivation in tissue culture of a cytopathogenic agent recovered from tissues of cattle with mucosal disease from 2 separated herds is reported. A homologous tissue culture system was used with bovine embryonic kidney cells and newborn, antibody-free calf serum. The virus was inactivated by temperatures above 50°C for 15 minutes, would pass an 03 Selas filter and had an 11th passage titer of 10-5. In sheep, the virus stimulated formation of neutralizing antibodies. A preliminary neutralization study indicated that antibodies are common in animals from herds having no previous history of mucosal disease.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-94-23091</identifier><identifier>PMID: 13431960</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Cattle ; Immunologic Tests ; Old Medline ; Pestivirus Infections ; Viruses - ethnology</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1957-04, Vol.94 (4), p.795-797</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-2a48554cb291170c0885a226257b33646d5963a1059c1f41d162df65836ff57d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13431960$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Underdahl, N. R.</creatorcontrib><creatorcontrib>Grace, O. D.</creatorcontrib><creatorcontrib>Hoerlein, A. B.</creatorcontrib><title>Cultivation in Tissue-Culture of Cytopathogenic Agent from Bovine Mucosal Disease.</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
Isolation and cultivation in tissue culture of a cytopathogenic agent recovered from tissues of cattle with mucosal disease from 2 separated herds is reported. A homologous tissue culture system was used with bovine embryonic kidney cells and newborn, antibody-free calf serum. The virus was inactivated by temperatures above 50°C for 15 minutes, would pass an 03 Selas filter and had an 11th passage titer of 10-5. In sheep, the virus stimulated formation of neutralizing antibodies. A preliminary neutralization study indicated that antibodies are common in animals from herds having no previous history of mucosal disease.</description><subject>Animals</subject><subject>Cattle</subject><subject>Immunologic Tests</subject><subject>Old Medline</subject><subject>Pestivirus Infections</subject><subject>Viruses - ethnology</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1957</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAURC0EoqXwAWyQV-xSfP1KvITylIqQUFlbbuIUV0lc7KRS_56EFrFjNdK9Z0aaQegSyJRBBjeEsFSlNE0UTygjCo7QGAQTCZNKHaPx8E8GYITOYlwTAiKl8hSNgHEGSpIxep91Veu2pnW-wa7BCxdjZ5Ph2gWLfYlnu9ZvTPvpV7ZxOb7tpcVl8DW-81vXWPza5T6aCt-7aE2003N0Upoq2ouDTtDH48Ni9pzM355eZrfzJOeEtgk1PBOC50uqAFKSkywThlJJRbpkTHJZCCWZASJUDiWHAiQtSikyJstSpAWboOt97ib4r87GVtcu5raqTGN9F3UGivWFRQ_CHsyDjzHYUm-Cq03YaSB6GFL_DqkV1z9D9p6rQ3i3rG3x5zgs1wPTPRDNyuq170LTl_0n8RsSHHnM</recordid><startdate>195704</startdate><enddate>195704</enddate><creator>Underdahl, N. R.</creator><creator>Grace, O. D.</creator><creator>Hoerlein, A. B.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>195704</creationdate><title>Cultivation in Tissue-Culture of Cytopathogenic Agent from Bovine Mucosal Disease.</title><author>Underdahl, N. R. ; Grace, O. D. ; Hoerlein, A. B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-2a48554cb291170c0885a226257b33646d5963a1059c1f41d162df65836ff57d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1957</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Immunologic Tests</topic><topic>Old Medline</topic><topic>Pestivirus Infections</topic><topic>Viruses - ethnology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Underdahl, N. R.</creatorcontrib><creatorcontrib>Grace, O. D.</creatorcontrib><creatorcontrib>Hoerlein, A. B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Underdahl, N. R.</au><au>Grace, O. D.</au><au>Hoerlein, A. B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cultivation in Tissue-Culture of Cytopathogenic Agent from Bovine Mucosal Disease.</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1957-04</date><risdate>1957</risdate><volume>94</volume><issue>4</issue><spage>795</spage><epage>797</epage><pages>795-797</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
Isolation and cultivation in tissue culture of a cytopathogenic agent recovered from tissues of cattle with mucosal disease from 2 separated herds is reported. A homologous tissue culture system was used with bovine embryonic kidney cells and newborn, antibody-free calf serum. The virus was inactivated by temperatures above 50°C for 15 minutes, would pass an 03 Selas filter and had an 11th passage titer of 10-5. In sheep, the virus stimulated formation of neutralizing antibodies. A preliminary neutralization study indicated that antibodies are common in animals from herds having no previous history of mucosal disease.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>13431960</pmid><doi>10.3181/00379727-94-23091</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1957-04, Vol.94 (4), p.795-797 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81931575 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cattle Immunologic Tests Old Medline Pestivirus Infections Viruses - ethnology |
| title | Cultivation in Tissue-Culture of Cytopathogenic Agent from Bovine Mucosal Disease. |
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