Cultivation in Tissue-Culture of Cytopathogenic Agent from Bovine Mucosal Disease.

Summary Isolation and cultivation in tissue culture of a cytopathogenic agent recovered from tissues of cattle with mucosal disease from 2 separated herds is reported. A homologous tissue culture system was used with bovine embryonic kidney cells and newborn, antibody-free calf serum. The virus was inactivated by temperatures above 50°C for 15 minutes, would pass an 03 Selas filter and had an 11th passage titer of 10-5. In sheep, the virus stimulated formation of neutralizing antibodies. A preliminary neutralization study indicated that antibodies are common in animals from herds having no previous history of mucosal disease.