Microdetermination of Acetylable Steroids in Plasma.

Summary A method is described which employs a combination of paper chromatographic and radioactive isotope technics. It is applicable to a wide number of hydroxylated steroids and is specific and sensitive to less than 0.1 μg quantities of steroids. The sensitivity is dependent upon the number of hydroxyl groups and specific activity of the radioactive acetic anhydride which is used to label the steroid. It has been applied successfully to the analysis of steroids in blood, urine, and a variety of tissues.