Automated determination of enzymatic rate functions by the ‘gradient-flow’ method
Under optimal experimental conditions a gradient-flow method has been employed to obtain an automated recording of the velocity-substrate relationship for the enzyme horse liver alcohol dehydrogenase. Distortions due to mechanical factors as well as due to excessive reaction could be eliminated. A g...
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| Veröffentlicht in: | Analytical biochemistry 1973-02, Vol.51 (2), p.584-588 |
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| container_end_page | 588 |
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| container_issue | 2 |
| container_start_page | 584 |
| container_title | Analytical biochemistry |
| container_volume | 51 |
| creator | Gurr, Patricia A. Tze-Fei Wong, J. Hanes, Charles S. |
| description | Under optimal experimental conditions a gradient-flow method has been employed to obtain an automated recording of the velocity-substrate relationship for the enzyme horse liver alcohol dehydrogenase. Distortions due to mechanical factors as well as due to excessive reaction could be eliminated. A general criterion for safeguarding the validity of rate functions recorded by this method has been proposed. |
| doi_str_mv | 10.1016/0003-2697(73)90514-9 |
| format | Article |
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Distortions due to mechanical factors as well as due to excessive reaction could be eliminated. A general criterion for safeguarding the validity of rate functions recorded by this method has been proposed.</description><subject>Alcohol Oxidoreductases</subject><subject>Animals</subject><subject>Biochemistry - instrumentation</subject><subject>Ethanol</subject><subject>Horses</subject><subject>Kinetics</subject><subject>Liver - enzymology</subject><subject>Mathematics</subject><subject>Methods</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLtOxDAQRS0EguXxByC5QlAExnEcJw0SQrwkJBqoLa89AaNNDLYDWio-A36PLyFhV5RUU9xzZzSHkF0GRwxYeQwAPMvLWh5IfliDYEVWr5AJg7rMgEO9SiZ_yAbZjPEJgLFClOtkvZBcCCEn5P60T77VCS21mDC0rtPJ-Y76hmL3Ph8iZ2gYANr0nRmjSKdzmh6Rfn98PgRtHXYpa2b-7fvji7aYHr3dJmuNnkXcWc4tcn9xfnd2ld3cXl6fnd5khguZMsEYgiwYcrCCVwKEblDkQktZ8WmBNi9LDUZCibJotLUoeV5VObAiN4g53yL7i73Pwb_0GJNqXTQ4m-kOfR9VxaqyFvUIFgvQBB9jwEY9B9fqMFcM1GhTjarUqEpJrn5tqnqo7S3399MW7V9pqW_ITxY5Dk--OgwqmkGHQesCmqSsd_8f-AGVqoW4</recordid><startdate>197302</startdate><enddate>197302</enddate><creator>Gurr, Patricia A.</creator><creator>Tze-Fei Wong, J.</creator><creator>Hanes, Charles S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197302</creationdate><title>Automated determination of enzymatic rate functions by the ‘gradient-flow’ method</title><author>Gurr, Patricia A. ; Tze-Fei Wong, J. ; Hanes, Charles S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-511e0741e30d538505afe525a7783b4ed266a0c706e74fadde7328820142cee23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Alcohol Oxidoreductases</topic><topic>Animals</topic><topic>Biochemistry - instrumentation</topic><topic>Ethanol</topic><topic>Horses</topic><topic>Kinetics</topic><topic>Liver - enzymology</topic><topic>Mathematics</topic><topic>Methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gurr, Patricia A.</creatorcontrib><creatorcontrib>Tze-Fei Wong, J.</creatorcontrib><creatorcontrib>Hanes, Charles S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gurr, Patricia A.</au><au>Tze-Fei Wong, J.</au><au>Hanes, Charles S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Automated determination of enzymatic rate functions by the ‘gradient-flow’ method</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1973-02</date><risdate>1973</risdate><volume>51</volume><issue>2</issue><spage>584</spage><epage>588</epage><pages>584-588</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Under optimal experimental conditions a gradient-flow method has been employed to obtain an automated recording of the velocity-substrate relationship for the enzyme horse liver alcohol dehydrogenase. 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| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1973-02, Vol.51 (2), p.584-588 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81869592 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alcohol Oxidoreductases Animals Biochemistry - instrumentation Ethanol Horses Kinetics Liver - enzymology Mathematics Methods |
| title | Automated determination of enzymatic rate functions by the ‘gradient-flow’ method |
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