Automated determination of enzymatic rate functions by the ‘gradient-flow’ method

Automated determination of enzymatic rate functions by the ‘gradient-flow’ method

Under optimal experimental conditions a gradient-flow method has been employed to obtain an automated recording of the velocity-substrate relationship for the enzyme horse liver alcohol dehydrogenase. Distortions due to mechanical factors as well as due to excessive reaction could be eliminated. A g...

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Veröffentlicht in:Analytical biochemistry 1973-02, Vol.51 (2), p.584-588
Hauptverfasser: Gurr, Patricia A., Tze-Fei Wong, J., Hanes, Charles S.
Format: Artikel
Sprache:eng
Schlagworte:
Alcohol Oxidoreductases
Animals
Biochemistry - instrumentation
Ethanol
Horses
Kinetics
Liver - enzymology
Mathematics
Methods
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Zusammenfassung:Under optimal experimental conditions a gradient-flow method has been employed to obtain an automated recording of the velocity-substrate relationship for the enzyme horse liver alcohol dehydrogenase. Distortions due to mechanical factors as well as due to excessive reaction could be eliminated. A general criterion for safeguarding the validity of rate functions recorded by this method has been proposed.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(73)90514-9