Growth of Bovine Amniotic Ectoderm as a Cell Monolayer on Glass and its use as a System for Virus Multiplication

IT has been found in this laboratory that monolayer cultures of cells on glass can be prepared successfully from bovine amniotic membranes. Such cultures have proved to be suitable for differential growth-curve experiments with the virus of foot-and-mouth disease in which frequent changing of the medium is necessary. The yields of virus obtained were essentially similar to those obtained with cell suspensions or monolayer cultures derived from adult bovine tongue tissue. This success, and that of Zitser et al. 1 with similar monolayer cell cultures from human amnion in growing the virus of poliomyelitis, suggest the more extended use of fœtal membranes for the study of mammalian viruses, and particularly those with a very restricted host or tissue susceptibility.