A novel real-time PCR method based on signaling-by-incorporation

We describe a novel real-time PCR method, which utilizes the in situ generated signal upon incorporation of a fluorogenic nucleotide. The method has been compared with a conventional method and evaluated for practical uses such as quantification of cDNA and malaria diagnosis.

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2011-01, Vol.47 (2), p.791-793
Hauptverfasser:	Kim, Da-Rae, Ahn, Hee-Chul, Lee, Won-Ja, Ahn, Dae-Ro
Format:	Artikel
Sprache:	eng
Schlagworte:	Deoxyguanine Nucleotides - chemistry Fluorine - chemistry Humans Malaria - diagnosis Plasmodium vivax - genetics Polymerase Chain Reaction - methods RNA, Ribosomal, 18S - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We describe a novel real-time PCR method, which utilizes the in situ generated signal upon incorporation of a fluorogenic nucleotide. The method has been compared with a conventional method and evaluated for practical uses such as quantification of cDNA and malaria diagnosis.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c0cc04516k