Influence of host reproductive state on Sphaerothecum destruens prevalence and infection level

Sphaerothecum destruens is an obligate intracellular parasite with the potential to cause high mortalities and spawning inhibition in the endangered cyprinid Leucaspius delineatus. We investigated the influence of L. delineatus’s reproductive state on the prevalence and infection level of S. destrue...

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Veröffentlicht in:	Parasitology 2011-01, Vol.138 (1), p.26-34
Hauptverfasser:	ANDREOU, D., HUSSEY, M., GRIFFITHS, S. W., GOZLAN, R. E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agnatha. Pisces Animal and plant ecology Animal, plant and microbial ecology Animals Biological and medical sciences Cohabitation Cyprinidae - parasitology Cyprinidae - physiology Deoxyribonucleic acid DNA Female Fish Diseases - epidemiology Fish Diseases - parasitology Fresh water ecosystems Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Invertebrates Male Mesomycetozoea - genetics Mesomycetozoea - physiology Mesomycetozoea Infections - epidemiology Mesomycetozoea Infections - parasitology Parasites Polymerase Chain Reaction - methods Prevalence Reproduction Spawning Synecology Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
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Zusammenfassung:	Sphaerothecum destruens is an obligate intracellular parasite with the potential to cause high mortalities and spawning inhibition in the endangered cyprinid Leucaspius delineatus. We investigated the influence of L. delineatus’s reproductive state on the prevalence and infection level of S. destruens. A novel real time quantitative polymerarse chain reaction (qPCR) was developed to determine S. destruens’ prevalence and infection level. These parameters were quantified and compared in reproductive and non-reproductive L. delineatus. The detection limit of the S. destruens specific qPCR was determined to be 1 pg of purified S. destruens genomic DNA. Following cohabitation in the lab, reproductive L. delineatus had a significantly higher S. destruens prevalence (P
ISSN:	0031-1820 1469-8161
DOI:	10.1017/S0031182010000983