Nucleoprotein fractions from various embryonic tissues: A comparison of physicochemical characteristics and biological activity in tissue culture

Nucleoprotein fractions from various embryonic tissues: A comparison of physicochemical characteristics and biological activity in tissue culture

1. 1. A nucleoprotein fraction (NPF) was isolated by streptomycin precipitation from twelve different chick embryo tissues. 2. 2. Bioassays in tissue culture revealed maximum growth-promoting activity in those NPF's from material containing cartilage. 3. 3. Electrophoretic analyses indicate tha...

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Veröffentlicht in:Experimental cell research 1956-01, Vol.10 (1), p.48-54
Hauptverfasser: Kutsky, R.J., Trautman, R., Lieberman, M., Cailleau, R.M.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Embryo, Mammalian - metabolism
Embryo, Nonmammalian
Nucleoproteins - metabolism
Old Medline
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Zusammenfassung:1. 1. A nucleoprotein fraction (NPF) was isolated by streptomycin precipitation from twelve different chick embryo tissues. 2. 2. Bioassays in tissue culture revealed maximum growth-promoting activity in those NPF's from material containing cartilage. 3. 3. Electrophoretic analyses indicate that the leading peaks of the highly active NPF's are much greater than those of the other NPF's. 4. 4. Ultraviolet analyses of tissue NPF's indicate similar nucleoprotein absorption spectra, while the electrophoretic subfractions of these NPF's exhibit families of curves which are similar and have maxima from 257 mμ to 275 mμ.
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(56)90069-6