The Rapid Chromatographic Analysis of the Free Nucleotides from Rat Brain

The acid soluble nucleotides of rat brains were analyzed by high pressure pellicular anion exchange chromatography. The method uses a linear concentration gradient formed by pumping 0.25 M KH2PO4 plus 2.2 M KCl at a rate of 12 ml per hour into a gradient mixer containing 45 ml of 0.01 M KH2PO4 plus...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of chromatographic science 1972-01, Vol.10 (1), p.38-40
1. Verfasser:	Shmukler, H W
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine Nucleotides - analysis Adenine Nucleotides - metabolism Adenosine Triphosphate - analysis Animals Autoanalysis Brain - metabolism Brain Chemistry Chromatography, Ion Exchange Cyclic AMP - analysis Hydrogen-Ion Concentration Male Methods Pressure Rats Solubility
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The acid soluble nucleotides of rat brains were analyzed by high pressure pellicular anion exchange chromatography. The method uses a linear concentration gradient formed by pumping 0.25 M KH2PO4 plus 2.2 M KCl at a rate of 12 ml per hour into a gradient mixer containing 45 ml of 0.01 M KH2PO4 plus 0.001 M H3PO4. The carrier flow rate was 24 ml/hr at 70° and 800 PSI. Fourteen nucleotides were separated from 1.0 mg of rat brain in 50 minutes. The mean composition and standard deviations for the acid soluble nucleotide composition of rat brain is given.
ISSN:	0021-9665 1945-239X
DOI:	10.1093/chromsci/10.1.38