Serial Propagation of 3 Strains of Rabbit Fibroblasts; Their Susceptibility to Infection with Vaccinia Virus.
Summary Twenty-three strains of fibroblasts derived from normal rabbit and chick embryo tissue were serially propagated on glass in a variety of media. All except 3 strains failed to proliferate for more than 8 to 12 subcultures. The 3 successful strains were derived from adult testis, embryonic ski...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1956-11, Vol.93 (2), p.200-204 |
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| container_end_page | 204 |
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| container_issue | 2 |
| container_start_page | 200 |
| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 93 |
| creator | Haff, R. F. Swim, H. E. |
| description | Summary
Twenty-three strains of fibroblasts derived from normal rabbit and chick embryo tissue were serially propagated on glass in a variety of media. All except 3 strains failed to proliferate for more than 8 to 12 subcultures. The 3 successful strains were derived from adult testis, embryonic skin-muscle and adult muscle respectively and continue to proliferate at a constant rate after 38 to 61 subcultures. Vaccinia virus multiplies in all 3 strains of fibroblasts resulting in the complete destruction of cells. |
| doi_str_mv | 10.3181/00379727-93-22707 |
| format | Article |
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Twenty-three strains of fibroblasts derived from normal rabbit and chick embryo tissue were serially propagated on glass in a variety of media. All except 3 strains failed to proliferate for more than 8 to 12 subcultures. The 3 successful strains were derived from adult testis, embryonic skin-muscle and adult muscle respectively and continue to proliferate at a constant rate after 38 to 61 subcultures. Vaccinia virus multiplies in all 3 strains of fibroblasts resulting in the complete destruction of cells.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-93-22707</identifier><identifier>PMID: 13379462</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Disease Susceptibility ; Fibroblasts ; Old Medline ; Rabbits ; Reproduction ; Vaccinia ; Vaccinia virus</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1956-11, Vol.93 (2), p.200-204</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-5db4e446aa748e7545733385680187d8733e9a351ee7c2f86640f0370894ea293</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13379462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haff, R. F.</creatorcontrib><creatorcontrib>Swim, H. E.</creatorcontrib><title>Serial Propagation of 3 Strains of Rabbit Fibroblasts; Their Susceptibility to Infection with Vaccinia Virus.</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
Twenty-three strains of fibroblasts derived from normal rabbit and chick embryo tissue were serially propagated on glass in a variety of media. All except 3 strains failed to proliferate for more than 8 to 12 subcultures. The 3 successful strains were derived from adult testis, embryonic skin-muscle and adult muscle respectively and continue to proliferate at a constant rate after 38 to 61 subcultures. Vaccinia virus multiplies in all 3 strains of fibroblasts resulting in the complete destruction of cells.</description><subject>Animals</subject><subject>Disease Susceptibility</subject><subject>Fibroblasts</subject><subject>Old Medline</subject><subject>Rabbits</subject><subject>Reproduction</subject><subject>Vaccinia</subject><subject>Vaccinia virus</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1956</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9PwjAYhxujEUQ_gBfTk7dh_23t4skQURISjSDXphsdlIwV2y6Gb28HGG-emjfv83vS9wfALUZDigV-QIjynBOe5DQhhCN-Bvo4pWlCszw_B_1un3RAD1x5v0EIp5xkl6CHaQyyjPTBdqadUTV8d3anVioY20BbQQpnwSnT-G74UEVhAhybwtmiVj74Rzhfa-PgrPWl3gVTmNqEPQwWTppKlwfLtwlruFBlaRqj4MK41g-vwUWlaq9vTu8AfI6f56PXZPr2Mhk9TZOSUhGSdFkwzVimFGdC85SlnMZFmgmEBV-KOOlc0RRrzUtSiSxjqIq3IpEzrUhOB-D-6N05-9VqH-TWxJ_WtWq0bb0UOIoJYRHER7B01nunK7lzZqvcXmIku47lb8cyp_LQcczcneRtsdXLv8Sp1AgMj4BXKy03tnVNPPYf4w_EwIOZ</recordid><startdate>195611</startdate><enddate>195611</enddate><creator>Haff, R. F.</creator><creator>Swim, H. E.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>195611</creationdate><title>Serial Propagation of 3 Strains of Rabbit Fibroblasts; Their Susceptibility to Infection with Vaccinia Virus.</title><author>Haff, R. F. ; Swim, H. E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-5db4e446aa748e7545733385680187d8733e9a351ee7c2f86640f0370894ea293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1956</creationdate><topic>Animals</topic><topic>Disease Susceptibility</topic><topic>Fibroblasts</topic><topic>Old Medline</topic><topic>Rabbits</topic><topic>Reproduction</topic><topic>Vaccinia</topic><topic>Vaccinia virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haff, R. F.</creatorcontrib><creatorcontrib>Swim, H. E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haff, R. F.</au><au>Swim, H. E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serial Propagation of 3 Strains of Rabbit Fibroblasts; Their Susceptibility to Infection with Vaccinia Virus.</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1956-11</date><risdate>1956</risdate><volume>93</volume><issue>2</issue><spage>200</spage><epage>204</epage><pages>200-204</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
Twenty-three strains of fibroblasts derived from normal rabbit and chick embryo tissue were serially propagated on glass in a variety of media. All except 3 strains failed to proliferate for more than 8 to 12 subcultures. The 3 successful strains were derived from adult testis, embryonic skin-muscle and adult muscle respectively and continue to proliferate at a constant rate after 38 to 61 subcultures. Vaccinia virus multiplies in all 3 strains of fibroblasts resulting in the complete destruction of cells.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>13379462</pmid><doi>10.3181/00379727-93-22707</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1956-11, Vol.93 (2), p.200-204 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81748224 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Disease Susceptibility Fibroblasts Old Medline Rabbits Reproduction Vaccinia Vaccinia virus |
| title | Serial Propagation of 3 Strains of Rabbit Fibroblasts; Their Susceptibility to Infection with Vaccinia Virus. |
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