Serial Propagation of 3 Strains of Rabbit Fibroblasts; Their Susceptibility to Infection with Vaccinia Virus.
Summary Twenty-three strains of fibroblasts derived from normal rabbit and chick embryo tissue were serially propagated on glass in a variety of media. All except 3 strains failed to proliferate for more than 8 to 12 subcultures. The 3 successful strains were derived from adult testis, embryonic ski...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1956-11, Vol.93 (2), p.200-204 |
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| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
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| Online-Zugang: | Volltext |
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| Zusammenfassung: | Summary
Twenty-three strains of fibroblasts derived from normal rabbit and chick embryo tissue were serially propagated on glass in a variety of media. All except 3 strains failed to proliferate for more than 8 to 12 subcultures. The 3 successful strains were derived from adult testis, embryonic skin-muscle and adult muscle respectively and continue to proliferate at a constant rate after 38 to 61 subcultures. Vaccinia virus multiplies in all 3 strains of fibroblasts resulting in the complete destruction of cells. |
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| ISSN: | 0037-9727 1535-3702 1535-3699 |
| DOI: | 10.3181/00379727-93-22707 |