Gas Chromatographic Determination of 3, 4-Dihydroxyphenylalanine (DOPA) in Urine

Gas Chromatographic Determination of 3, 4-Dihydroxyphenylalanine (DOPA) in Urine

Urinary 3, 4-dihydroxyphenylalanine (DOPA) was extracted with alumina after removal of the fatty substances by shaking with ether-benzene (5 : 2), converted to n-butyl ester with n-butanol-HCl (5N) at 100°for 20 min, trifluoroacetylated with trifluoroacetic anhydride in ethyl acetate and determined...

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Veröffentlicht in:Chemical & pharmaceutical bulletin 1972/11/25, Vol.20(11), pp.2436-2439
Hauptverfasser: IMAI, KAZUHIRO, ARIZUMI, NOBUKO, WANG, MEITAI, YOSHIUE, SHOHEI, TAMURA, ZENZO
Format: Artikel
Sprache:eng
Schlagworte:
Chromatography, Gas
Dihydroxyphenylalanine - urine
Humans
Mass Spectrometry
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Zusammenfassung:Urinary 3, 4-dihydroxyphenylalanine (DOPA) was extracted with alumina after removal of the fatty substances by shaking with ether-benzene (5 : 2), converted to n-butyl ester with n-butanol-HCl (5N) at 100°for 20 min, trifluoroacetylated with trifluoroacetic anhydride in ethyl acetate and determined by gas chromatography using electron capture detector. The overall recoveries of the added DOPA (50, 75, 100, and 150 ng) from urine (2 ml) were 66.0% ±3.55% (n=8). The pressence of DOPA in normal human urine was confirmed by gas chromatographymass spectroscopy.
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.20.2436