Determination of blood kinin levels by radioimmunoassay

Determination of blood kinin levels by radioimmunoassay

A radioimmunoassay has been developed which enables measurement of kinin levels in blood of man and dogs. The levels found are not due to production of kinin by kallikrein activation during collection. The material detected by this assay is immunologically similar to synthetic bradykinin (BK) and ha...

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Veröffentlicht in:Biochemical pharmacology 1972-10, Vol.21 (20), p.2727-2735
Hauptverfasser: Mashford, M.L., Roberts, M.L.
Format: Artikel
Sprache:eng
Schlagworte:
Adolescent
Adult
Animals
Bradykinin - blood
Carbon Isotopes
Carboxypeptidases - pharmacology
Chymotrypsin - pharmacology
Dogs
Female
Humans
Kinins - blood
Male
Middle Aged
Rabbits - immunology
Radioimmunoassay
Trypsin - pharmacology
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Beschreibung
Zusammenfassung:A radioimmunoassay has been developed which enables measurement of kinin levels in blood of man and dogs. The levels found are not due to production of kinin by kallikrein activation during collection. The material detected by this assay is immunologically similar to synthetic bradykinin (BK) and has the same susceptibility to proteolytic enzymes. Levels found in venous blood in man average 0·07 ng/ml (S.D. 0·03 ug/ml). In a dog receiving an infusion of synthetic BK the levels detected downstream from the infusion site rose progressively with increased dose. Dogs show a positive arterio-venous difference across the vascular bed of the hind limb.
ISSN:0006-2952
1873-2968
DOI:10.1016/0006-2952(72)90021-4