Quantitative, label-free and site-specific monitoring of molecular recognition: a multivariate resonance Raman approach

Quantitative, label-free and site-specific monitoring of molecular recognition: a multivariate resonance Raman approach

A site-specific and quantitative approach for label-free monitoring of molecular recognition is presented. Specifically, the binding site of an artificial receptor is probed selectively by UVRR spectroscopy. The ligand binding constant can be determined by non-negative matrix factorization.

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chemical communications (Cambridge, England) England), 2011-01, Vol.47 (1), p.568-570
Hauptverfasser: Niebling, Stephan, Kuchelmeister, Hannes Y, Schmuck, Carsten, Schlücker, Sebastian
Format: Artikel
Sprache:eng
Schlagworte:
Binding Sites
Carboxylic Acids - chemistry
Ligands
Oligopeptides - chemistry
Receptors, Peptide - chemistry
Spectrum Analysis, Raman
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A site-specific and quantitative approach for label-free monitoring of molecular recognition is presented. Specifically, the binding site of an artificial receptor is probed selectively by UVRR spectroscopy. The ligand binding constant can be determined by non-negative matrix factorization.
ISSN:1359-7345
1364-548X
DOI:10.1039/c0cc02052d