Comparative proteomic analysis of differentially expressed proteins in primary retinoblastoma tumors

Purpose: To understand the disease mechanism and to identify the potential tumor markers that would help in therapeutics, comparative proteomic analysis of 29 retinoblastoma (RB) tumors was performed using 14 non‐neoplastic retinas (age ranged from 45 to 89 years) as control tissues. Experimental de...

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Veröffentlicht in:	Proteomics. Clinical applications 2010-04, Vol.4 (4), p.449-463
Hauptverfasser:	Mallikarjuna, Kandalam, Sundaram, Curam Sreenivasacharlu, Sharma, Yogendra, Deepa, Perinkulam Ravi, Khetan, Vikas, Gopal, Lingam, Biswas, Jyotirmay, Sharma, Tarun, Krishnakumar, Subramanian
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Aged, 80 and over Biological and medical sciences Biomarkers, Tumor - blood Differential expression Diverse techniques Electrophoresis, Gel, Two-Dimensional Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Male Medical sciences Middle Aged Molecular and cellular biology Ophthalmology Proteomics Retina Retinoblastoma Retinoblastoma - metabolism Reverse Transcriptase Polymerase Chain Reaction Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tumors and pseudotumors of the eye, orbit, eyelid, lacrimal apparatus
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Zusammenfassung:	Purpose: To understand the disease mechanism and to identify the potential tumor markers that would help in therapeutics, comparative proteomic analysis of 29 retinoblastoma (RB) tumors was performed using 14 non‐neoplastic retinas (age ranged from 45 to 89 years) as control tissues. Experimental design: 2‐DE and MALDI‐TOF‐TOF MS/MS were used to identify differentially expressed proteins. Results: Twenty‐seven distinct differentially expressed proteins were identified, including 16 upregulated 11 downregulated proteins. Significantly, higher mRNA levels of apolipoprotein A1 (p
ISSN:	1862-8346 1862-8354
DOI:	10.1002/prca.200900069