Combining laser capture microdissection and proteomics: Methodologies and clinical applications

Laser capture microdissection (LCM) has become an important tool for biomedical research. Various molecular biology techniques have been combined with LCM to reveal molecular profiles at an unprecedented tissue resolution. Proteomics is among those techniques that have proved fruitful in combination...

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Veröffentlicht in:	Proteomics. Clinical applications 2010-02, Vol.4 (2), p.116-123
1. Verfasser:	Xu, Baogang J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biomedical Research - methods Chemistry Techniques, Analytical Clinical applications Diverse techniques Fundamental and applied biological sciences. Psychology Humans Laser capture microdissection Lasers Microdissection - methods Molecular and cellular biology Protein Array Analysis Protein arrays Proteomics Proteomics - methods
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Beschreibung
Zusammenfassung:	Laser capture microdissection (LCM) has become an important tool for biomedical research. Various molecular biology techniques have been combined with LCM to reveal molecular profiles at an unprecedented tissue resolution. Proteomics is among those techniques that have proved fruitful in combination with LCM. This review provides an overview of the various proteomic techniques that have been developed for analyzing cells obtained using LCM. Methods for analyzing microdissected cells for various proteomic techniques are described and compared. In addition, various applications based on LCM and proteomics are discussed.
ISSN:	1862-8346 1862-8354
DOI:	10.1002/prca.200900138