ON THE CHEMISTRY OF THE THIOCHOLINE METHOD OF KOELLE

ON THE CHEMISTRY OF THE THIOCHOLINE METHOD OF KOELLE

In the thiocholine method of Koelle as previously described (17), the enzymatic reaction step leads to the formation of crystalline deposits. These have been identified as Cu·thiocholine sulfate. The subsequent ammonium sulfide treatment aiming at the conversion of Cu·thiocholine to CuS implies that...

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Veröffentlicht in:The journal of histochemistry and cytochemistry 1955-11, Vol.3 (6), p.441-445
Hauptverfasser: MALMGREN, H, SYLVEN, B
Format: Artikel
Sprache:eng
Schlagworte:
Cholinesterases - analysis
Old Medline
Thiocholine
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Zusammenfassung:In the thiocholine method of Koelle as previously described (17), the enzymatic reaction step leads to the formation of crystalline deposits. These have been identified as Cu·thiocholine sulfate. The subsequent ammonium sulfide treatment aiming at the conversion of Cu·thiocholine to CuS implies that the previous crystals become dissolved and replaced by amorphous precipitates. The environmental and other operating factors in this multi-step method are discussed.
ISSN:0022-1554
1551-5044
DOI:10.1177/3.6.441