Fractionation of rat cutaneous glycosaminoglycans using an anion-exchange resin

1. 1. In the presence of dilute acetic acid, chondroitin 4-sulfate can be eluted from AG 1 anion-exchange resin separately from dermatan sulfate. 2. 2. This principle has been used as the basis of a simple method for the separation of the bulk of the hyaluronate and dermatan sulfate from the other g...

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Veröffentlicht in:	Analytical biochemistry 1972-11, Vol.50 (1), p.63-72
Hauptverfasser:	Pearce, R.H., Grimmer, B.J., Mathieson, J.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetates Animals Chondroitin - isolation & purification Chromatography, Ion Exchange Electrophoresis Evaluation Studies as Topic Glycosaminoglycans - analysis Glycosaminoglycans - isolation & purification Hyaluronic Acid - isolation & purification Male Methods Microchemistry Rats Skin - analysis Sulfuric Acids - isolation & purification Uronic Acids - analysis
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Zusammenfassung:	1. 1. In the presence of dilute acetic acid, chondroitin 4-sulfate can be eluted from AG 1 anion-exchange resin separately from dermatan sulfate. 2. 2. This principle has been used as the basis of a simple method for the separation of the bulk of the hyaluronate and dermatan sulfate from the other glycosaminoglycans of rat skin. 3. 3. The cutaneous glycosaminoglycans behave differently than reference glycosaminoglycans in this system.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(72)90486-1