Modifications of mitochondrial ATPase in chromosomal respiratory-deficient mutants of a “petite-negative” yeast: Schizosaccharomyces pombe 972h

6 stable mutants with modified mitochondrial ATPase activity were obtained from a petite-negative yeast Schizosaccharomyces pombe . All the mutants have lost oligomycin-sensitive ATPase. Compared to the wild strain, cells of the strains RD14, RD15, M126 and Res4 have lost about 75% of the particulate dio-9 sensitive ATPase activity. Two other strains: M53 and RD32 have lost more than 95% of the total dio-9 sensitive ATPase activity per cell. M126, Res4, M53 and RD15 were shown to be of chromosomal heredity. All mutants are pleiotropic and show various cytochrome deficiencies.