Simian Sarcoma Virus, Type 1 (Lagothrix): Focus Assay and Demonstration of Nontransforming Associated Virus

The focal transformation of marmoset lung cells by simian sarcoma virus, type 1 (SSV-1) was quantitative without an agar overlay required. Pretreatment of the indicator cells with diethylaminoethyl-dextran increased the sensitivity 10–50 times. A 10-100 fold excess of nontransforming associated viru...

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Veröffentlicht in:	JNCI : Journal of the National Cancer Institute 1972-06, Vol.48 (6), p.1905-1908
Hauptverfasser:	Wolfe, Lauren G., Smith, Richard K., Deinhardt, Friedrich
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological Assay Cell Transformation, Neoplastic Dextrans Haplorhini Oncogenic Viruses Sarcoma, Experimental - etiology Satellite Viruses
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Zusammenfassung:	The focal transformation of marmoset lung cells by simian sarcoma virus, type 1 (SSV-1) was quantitative without an agar overlay required. Pretreatment of the indicator cells with diethylaminoethyl-dextran increased the sensitivity 10–50 times. A 10-100 fold excess of nontransforming associated virus (SSAV-1) was demonstrated in SSV-1 stocks by interference tests and electron microscopy. SSV-1 produced by transformed marmoset, human, or cat cells retained its oncogenicity for marmosets.
ISSN:	0027-8874 1460-2105
DOI:	10.1093/jnci/48.6.1905