Evaluation of the Resistance of DNA Immobilized on Ferrimagnetic Particles of Cobalt Ferrite Nanopowder against Nuclease Cleavage

Evaluation of the Resistance of DNA Immobilized on Ferrimagnetic Particles of Cobalt Ferrite Nanopowder against Nuclease Cleavage

DNA was immobilized on ferrimagnetic particles of cobalt ferrite nanopowder (CoFe 2 O 4 ) and its resistance to endonuclease (DNase I) hydrolysis was studied. Immobilization on cobalt ferrite nanoparticles prevented enzymatic cleavage of DNA. This process was not associated with enzyme inactivation...

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Veröffentlicht in:Bulletin of experimental biology and medicine 2010-07, Vol.149 (1), p.67-69
Hauptverfasser: Pershina, A. G., Sazonov, A. E., Ogorodova, L. M.
Format: Artikel
Sprache:eng
Schlagworte:
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cobalt
Cobalt - chemistry
Deoxyribonuclease I - metabolism
DNA
DNA - chemistry
DNA - metabolism
Electrophoresis, Agar Gel
Enzymes
Genetic research
Hydrolysis
Internal Medicine
Iron compounds
Laboratory Medicine
Magnetite Nanoparticles - chemistry
Nanotechnologies
Pathology
Spectrophotometry
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Zusammenfassung:DNA was immobilized on ferrimagnetic particles of cobalt ferrite nanopowder (CoFe 2 O 4 ) and its resistance to endonuclease (DNase I) hydrolysis was studied. Immobilization on cobalt ferrite nanoparticles prevented enzymatic cleavage of DNA. This process was not associated with enzyme inactivation under the effect of nanosize cobalt ferrite and was presumably determined by lesser availability of the DNA molecule as a result of its interaction with nanoparticles.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-010-0877-4