A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus membrane potential

We developed a homogenous microtiter based assay using the cationic dye 3, 3′-Diethyloxacarbocyanine iodide, DiOC 2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti- Escherichia coli act...

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Veröffentlicht in:	Journal of microbiological methods 2010-11, Vol.83 (2), p.254-256
Hauptverfasser:	Gentry, Daniel R., Wilding, Imogen, Johnson, John M., Chen, Dongzhao, Remlinger, Katja, Richards, Cindy, Neill, Susan, Zalacain, Magdalena, Rittenhouse, Stephen F., Gwynn, Michael N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology Assay Bacteriological methods and techniques used in bacteriology Bacteriological Techniques - methods Bacteriology Biological and medical sciences Carbocyanines - metabolism Escherichia coli - drug effects Fundamental and applied biological sciences. Psychology High-Throughput Screening Assays - methods Inhibitory Concentration 50 Membrane potential Membrane Potentials - drug effects Microbiology Microtiter Staphylococcus aureus Staphylococcus aureus - drug effects
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Zusammenfassung:	We developed a homogenous microtiter based assay using the cationic dye 3, 3′-Diethyloxacarbocyanine iodide, DiOC 2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti- Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2010.08.012