Novel Activity of Rhodobacter sphaeroides Spheroidene Monooxygenase CrtA Expressed in Escherichia coli

The spheroidene monooxygenase CrtA of Rhodobacter sphaeroides introduces a keto group and/or hydroxy group at the ends of nonnative substrates in Escherichia coli, resulting in the production of novel oxocarotenoids. The heme-containing CrtA is not a P450 enzyme but a new type of oxygenase.

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Applied and Environmental Microbiology 2010-11, Vol.76 (21), p.7328-7331
Hauptverfasser:	Lee, Pyung Cheon, Holtzapple, Erik, Schmidt-Dannert, Claudia
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Biological and medical sciences Biotechnology Carotenoids - isolation & purification Carotenoids - metabolism Chromatography, Thin Layer Crystallography, X-Ray E coli Enzymes Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene expression Microbiology Mixed Function Oxygenases - genetics Mixed Function Oxygenases - metabolism Organisms, Genetically Modified - genetics Organisms, Genetically Modified - metabolism Proteins Rhodobacter sphaeroides Rhodobacter sphaeroides - enzymology Rhodobacter sphaeroides - genetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The spheroidene monooxygenase CrtA of Rhodobacter sphaeroides introduces a keto group and/or hydroxy group at the ends of nonnative substrates in Escherichia coli, resulting in the production of novel oxocarotenoids. The heme-containing CrtA is not a P450 enzyme but a new type of oxygenase.
ISSN:	0099-2240 1098-5336 1098-6596
DOI:	10.1128/AEM.01606-10