Fabrication of Neisseria gonorrhoeae biosensor based on chitosan–MWCNT platform
We report results of studies relating to preparation and characterization of DNA electrodes based on the chitosan–MWCNT/ITO nanobiocomposite platform for electrochemical detection of gonorrhoea, the most common bacterial sexually transmitted disease. (STD). This biosensing electrode has been charact...
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Veröffentlicht in: | Thin solid films 2010-11, Vol.519 (3), p.1135-1140 |
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Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
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Zusammenfassung: | We report results of studies relating to preparation and characterization of DNA electrodes based on the chitosan–MWCNT/ITO nanobiocomposite platform for electrochemical detection of gonorrhoea, the most common bacterial sexually transmitted disease. (STD). This biosensing electrode has been characterized using FT-IR, SEM, cyclic voltammetry and differential pulse voltammetry, etc. The sensing characteristics have been investigated in phosphate buffer saline using methylene blue as the hybridization indicator. This DNA biosensor has a response time of 60
s and is found to be stable for about 4
months when stored at 4
°C. The results of DPV studies reveal that this bioelectrode can detect complementary DNA concentration in a wide range of 1
×
10
−
6
M to 1
×
10
−
17
M with a detection limit of 1
×
10
−
16
M. |
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ISSN: | 0040-6090 1879-2731 |
DOI: | 10.1016/j.tsf.2010.08.057 |