Enhancement of acid tolerance in Zymomonas mobilis by a proton-buffering peptide

A portion of the cbpA gene from Escherichia coli K-12 encoding a 24 amino acid proton-buffering peptide (Pbp) was cloned via the shuttle vector pJB99 into E. coli JM105 and subsequently into Zymomonas mobilis CP4. Expression of Pbp was confirmed in both JM105 and CP4 by HPLC. Z. mobilis CP4 carrying...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2006-07, Vol.134 (1), p.15-26
Hauptverfasser:	Baumler, D.J, Hung, K.F, Bose, J.L, Vykhodets, B.M, Cheng, C.M, Jeong, K.C, Kaspar, C.W
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetic acid acid tolerance Amino Acid Sequence Amino acids ampicillin antibiotic resistance Base Sequence buffers Cloning DNA, Bacterial - genetics E coli Escherichia coli Escherichia coli K12 - genetics Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism ethanol production Genes Genes, Bacterial genetic engineering Genetic Vectors Hydrogen-Ion Concentration industrial microbiology Liquid chromatography microbial growth molecular cloning Molecular Sequence Data Organic acids Peptide Fragments - genetics Peptide Fragments - metabolism Peptides Plasmids - genetics Protons recombinant proteins structure-activity relationships Studies Transformation, Genetic Zymomonas - genetics Zymomonas - growth & development Zymomonas - metabolism Zymomonas mobilis
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Zusammenfassung:	A portion of the cbpA gene from Escherichia coli K-12 encoding a 24 amino acid proton-buffering peptide (Pbp) was cloned via the shuttle vector pJB99 into E. coli JM105 and subsequently into Zymomonas mobilis CP4. Expression of Pbp was confirmed in both JM105 and CP4 by HPLC. Z. mobilis CP4 carrying pJB99-2 (Pbp) exhibited increased acid tolerance (p
ISSN:	0273-2289 1559-0291 0273-2289
DOI:	10.1385/ABAB:134:1:15