In vitro activity of isavuconazole against 140 reference fungal strains and 165 clinically isolated yeasts from Japan

Abstract The in vitro susceptibilities of 140 laboratory reference strains of fungi, including type strains, and 165 clinical yeast isolates from Japan towards isavuconazole compared with fluconazole (FLC), itraconazole (ITC), voriconazole and amphotericin B were measured. Broth microdilution methods based on Clinical and Laboratory Standards Institute (CLSI) methods were used for yeasts, and RPMI-MOPS medium semi-solidified with 0.2% low-melting-point agarose based on CLSI guidelines was used for moulds. The range of isavuconazole minimum inhibitory concentrations (MICs) was 0.0004–0.21 mg/L for Candida albicans , 0.0036–0.4 mg/L for Candida glabrata , 0.023–0.058 mg/L for Candida krusei , 0.0026–0.032 mg/L for Cryptococcus neoformans , 0.1–0.39 mg/L for Aspergillus fumigatus and 0.2–0.39 mg/L for Aspergillus terreus . Isavuconazole was as active as ITC against the dimorphic true pathogenic fungi, with a range of MICs from