Polyriboadenylate polymerase solubilized from rat liver mitochondria

Polyriboadenylate polymerase solubilized from rat liver mitochondria

An enzyme that can synthesize polyriboadenylate chains from ATP has been solubilized from rat liver mitochondria and characterized. The enzyme incorporates AMP residues into the internucleotide linkage. It is insensitive to the usual inhibitors of RNA polymerases. In the native state, poly (A) polym...

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Veröffentlicht in:Biochemical and biophysical research communications 1972-07, Vol.48 (1), p.126-134
Hauptverfasser: Jacob, Samson T., Schindler, Daniel G.
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine Triphosphate
Ammonium Sulfate
Animals
Chemical Precipitation
Deoxyribonucleases - pharmacology
Ethidium - pharmacology
Mitochondria, Liver - drug effects
Mitochondria, Liver - enzymology
Mitochondrial Swelling
Nucleotides
Nucleotidyltransferases - antagonists & inhibitors
Nucleotidyltransferases - isolation & purification
Rats
Ribonucleases - pharmacology
Structure-Activity Relationship
Tritium
Vibration
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Zusammenfassung:An enzyme that can synthesize polyriboadenylate chains from ATP has been solubilized from rat liver mitochondria and characterized. The enzyme incorporates AMP residues into the internucleotide linkage. It is insensitive to the usual inhibitors of RNA polymerases. In the native state, poly (A) polymerase is associated with a nuclease that degrades the product. Solubilization of this enzyme from isolated mitochondria releases active enzyme from inhibitory action of the nuclease.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(72)90353-1