Cell-free protein synthesis with sodium dodecyl sulfate and ADP

Cell-free protein synthesis with sodium dodecyl sulfate and ADP

A procedure is described for increasing the amount of acid-precipitable polyphenylalanine obtained from crude S-30 extracts of E. coli MRE-600 using the poly U directed Nirenberg assay system. The method requires addition of SDS and ADP to the assay solution in addition to its normal constituents. U...

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Veröffentlicht in:Analytical biochemistry 1972-07, Vol.48 (1), p.292-299
Hauptverfasser: Töpert, M., Rammler, D.H.
Format: Artikel
Sprache:eng
Schlagworte:
Adenine Nucleotides - pharmacology
Adenosine Diphosphate - pharmacology
Bacterial Proteins - biosynthesis
Carbon Isotopes
Cell-Free System
Chemical Precipitation
Drug Stability
Escherichia coli - drug effects
Escherichia coli - metabolism
Fatty Alcohols - pharmacology
Hydrogen-Ion Concentration
Kinetics
Methods
Osmolar Concentration
Peptide Biosynthesis
Phenylalanine - metabolism
Phosphoenolpyruvate
Polynucleotides - metabolism
RNA
Sodium Dodecyl Sulfate - pharmacology
Surface-Active Agents - pharmacology
Uracil Nucleotides - metabolism
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Zusammenfassung:A procedure is described for increasing the amount of acid-precipitable polyphenylalanine obtained from crude S-30 extracts of E. coli MRE-600 using the poly U directed Nirenberg assay system. The method requires addition of SDS and ADP to the assay solution in addition to its normal constituents. Using this procedure, a 9- to 10-fold increase in acid-precipitable radioactivity is obtained in a 60 min assay period.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(72)90192-3