A method for fractionating the carbohydrate components of bone

A method for fractionating the carbohydrate components of bone

A simple method has been devised for separating the carbohydrate components of the organic framework of bone into two distinct fractions. The method involves alcohol fractionation of a neutralized sodium hydroxide extract. An acid mucopolysaccharide fraction was obtained from 63% alcohol (Fraction I...

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Veröffentlicht in:Archives of biochemistry and biophysics 1955-03, Vol.55 (1), p.19-24
Hauptverfasser: Glegg, R.E., Eidinger, D.
Format: Artikel
Sprache:eng
Schlagworte:
Bone and Bones
Carbohydrates - analysis
Chemical Fractionation
Humans
Old Medline
Sweetening Agents
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Zusammenfassung:A simple method has been devised for separating the carbohydrate components of the organic framework of bone into two distinct fractions. The method involves alcohol fractionation of a neutralized sodium hydroxide extract. An acid mucopolysaccharide fraction was obtained from 63% alcohol (Fraction I), and is tentatively considered to be chondroitin sulfate. A hitherto undescribed fraction was obtained at 84% alcohol (Fraction II) which contains fucose, galactose, mannose, and glucosamine. The method is capable of general application to other types of connective tissue derivatives.
ISSN:0003-9861
1096-0384
DOI:10.1016/0003-9861(55)90538-5