A HISTOCHEMICAL METHOD FOR THE DEMONSTRATION OF AMINOPEPTIDASE B ACTIVITY

A HISTOCHEMICAL METHOD FOR THE DEMONSTRATION OF AMINOPEPTIDASE B ACTIVITY

A histochemical method for the demonstration of aminopeptidase B activity is presented. The method is based on the specific activation of the enzyme by chloride ions present in physiologic concentrations (0.9% or 0.154 M) in the incubating medium, on the inhibition by phosphate ions and on the use o...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The journal of histochemistry and cytochemistry 1972-03, Vol.20 (3), p.192-194
Hauptverfasser: MAKINEN, KAUKO K, PAUNIO, KEIJO U
Format: Artikel
Sprache:eng
Schlagworte:
Amines
Aminopeptidases - analysis
Arginine
Chlorides - pharmacology
Enzyme Activation - drug effects
Gingiva - enzymology
Histocytochemistry
Humans
Naphthalenes
Phosphates
Staining and Labeling
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A histochemical method for the demonstration of aminopeptidase B activity is presented. The method is based on the specific activation of the enzyme by chloride ions present in physiologic concentrations (0.9% or 0.154 M) in the incubating medium, on the inhibition by phosphate ions and on the use of N-l-arginyl-2-naphthylamine as substrate. The liberated 2-naphthylamine was coupled with fast blue B.
ISSN:0022-1554
1551-5044
DOI:10.1177/20.3.192