P450Arom induction in isolated control endometrial cells by peritoneal fluid from women with endometriosis

To study the effect of peritoneal fluid from women with (PF-E) and without (PF-C) endometriosis on P450Arom expression in endometrial cells. Experimental study. University research unit. Forty women of reproductive age with (n = 22) or without (control; n = 18) endometriosis. Peritoneal fluid and eu...

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Veröffentlicht in:Fertility and sterility 2010-12, Vol.94 (7), p.2521-2527
Hauptverfasser: Castro, Jazmin, Torres, Marisa, Sovino, Hugo, Fuentes, Ariel, Boric, M. Angélica, Johnson, M. Cecilia
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Sprache:eng
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Zusammenfassung:To study the effect of peritoneal fluid from women with (PF-E) and without (PF-C) endometriosis on P450Arom expression in endometrial cells. Experimental study. University research unit. Forty women of reproductive age with (n = 22) or without (control; n = 18) endometriosis. Peritoneal fluid and eutopic endometrial samples were obtained during surgery from women with (n = 13 and 9, respectively) and without (n = 4 and 14, respectively) endometriosis. Expression study for P450Arom, steroid factor 1 (SF-1), chicken ovalbumin upstream transcription factor I (COUP-TFI), and COUP-TFII messenger RNA (reverse transcriptase–polymerase chain reacion) and/or protein (immunoblot) in isolated endometrial epithelial cells transfected or not with expression vector containing SF-1, COUP-TFI, or COUP-TFII complementary DNAs. Basal messenger RNA and/or protein expression of P450Arom and SF-1 were augmented in endometriosis, and that of COUP-TF was diminished. In control cells, (Bu)2cAMP and PF-E increased P450Arom and SF-1 expression (but not COUP-TF expression) in a dose-dependent way, an effect not observed with PF-C, adsorbed PF-E, or 10−5 M indomethacin. Transfected cells confirmed these results. Any treatments modified the studied molecules in endometriosis cells. These data indicate that molecules contained in PF-E favor an estrogenic microenvironment, suggesting a role in the etiopathogenesis of endometriosis enabling the survival, maintenance, and growth of endometrial implants in the ectopic locations.
ISSN:0015-0282
1556-5653
DOI:10.1016/j.fertnstert.2010.03.036