Polyvinylpyrrolidone (PVP) as Adjunct in Centrifugal Separation and Enzymic Assay of Subcellular Components.

Polyvinylpyrrolidone (PVP) as Adjunct in Centrifugal Separation and Enzymic Assay of Subcellular Components.

Summary Addition of polyvinylpyrrolidone (PVP) to sucrose solutions used in extraction of subcellular components improved centrifugal resolution of particulates, helped to preserve morphologic integrity of subcellular elements, and aided in preservation of enzymic activities. PVP was particularly us...

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Veröffentlicht in:Experimental biology and medicine (Maywood, N.J.) N.J.), 1954-10, Vol.87 (1), p.71-73
1. Verfasser: Woods, Mark W.
Format: Artikel
Sprache:eng
Schlagworte:
Biological Assay
Old Medline
Povidone
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Zusammenfassung:Summary Addition of polyvinylpyrrolidone (PVP) to sucrose solutions used in extraction of subcellular components improved centrifugal resolution of particulates, helped to preserve morphologic integrity of subcellular elements, and aided in preservation of enzymic activities. PVP was particularly useful in counteracting irreversible agglutination of microsomes
ISSN:0037-9727
1535-3702
1535-3699
DOI:10.3181/00379727-87-21288