Synthesis and properties of poly 2′-fluoro-2′-deoxyuridylic acid

Poly 2′-fluoro-2′-deoxyuridylic acid was synthesized by polymerization of the corresponding nucleoside-5′-diphosphate with polynucleotide phosphorylase from Escherichia coli. The polymer is resistant to degradation by pancreatic RNase and alkali. The polymer possesses no secondary structure but form...

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Veröffentlicht in:	Biochemical and biophysical research communications 1972-02, Vol.46 (3), p.1153-1160
Hauptverfasser:	Janik, B., Kotick, M.P., Kreiser, T.H., Reverman, L.F., Sommer, R.G., Wilson, D.P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine Nucleotides Chemical Phenomena Chemistry Chromatography, Gel Deoxyribonucleases Escherichia coli - enzymology Floxuridine Fluorine Hot Temperature Hydrogen-Ion Concentration Inosine Nucleotides Micrococcal Nuclease Nucleic Acid Conformation Nucleic Acid Denaturation Nucleic Acid Hybridization Nucleotidyltransferases Phosphoric Monoester Hydrolases Polynucleotides - chemical synthesis Ribonucleases RNA Spectrophotometry Spermidine Ultraviolet Rays Uracil Nucleotides - chemical synthesis
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Beschreibung
Zusammenfassung:	Poly 2′-fluoro-2′-deoxyuridylic acid was synthesized by polymerization of the corresponding nucleoside-5′-diphosphate with polynucleotide phosphorylase from Escherichia coli. The polymer is resistant to degradation by pancreatic RNase and alkali. The polymer possesses no secondary structure but forms a 1:1 complex with both poly A and poly X.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/S0006-291X(72)80095-0