Isolation of an Adenovirus 32 Strain from Human Brain in a Case of Subacute Encephalitis

Summary This report describes the isolation in tissue culture of an adenovirus from the brain of a 42-year-old man dying with radiation-treated lymphosarcoma and a 4.5-week history of neurological disease which, on histopathological evidence, was diagnosed as subacute encephalitis. The isolate, designated the JL strain, was most closely related antigenically to Ad. 32 by NT test and to Ad. 27 by HI test. Type-specific antisera to 31 additional adenoviruses failed to show significant neutralizing or hem-agglutination-inhibiting relationships. Similarly, antisera prepared in rabbits and monkeys inoculated with the JL strain neutralized only homologous virus and Ad. 32 and inhibited hemagglutination of the homologous virus and Ad. 27 to a significant titer.