Cysteine: A potential source of error in amino acid analysis of mercaptoethane sulfonic or hydrochloric acid hydrolysates of proteins and peptides
Hydrolysis of proteins and peptides with mercaptoethane sulfonic acid is liable to produce overestimation of the proline content owing to the production of ninhydrin-positive material (probably cysteine) which coelutes with proline on many ion-exchange analytical systems. A similar error occurs with...
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| Veröffentlicht in: | Analytical biochemistry 1984-01, Vol.141 (2), p.429-431 |
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| container_end_page | 431 |
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| container_title | Analytical biochemistry |
| container_volume | 141 |
| creator | Gardner, Michael L.G. |
| description | Hydrolysis of proteins and peptides with mercaptoethane sulfonic acid is liable to produce overestimation of the proline content owing to the production of ninhydrin-positive material (probably cysteine) which coelutes with proline on many ion-exchange analytical systems. A similar error occurs with HCl hydrolysis (especially in the presence of mercaptoethanol or thioglycollic acid) if care is not taken to oxidize cysteine during reconstitution of the hydrolysate before amino acid analysis. |
| doi_str_mv | 10.1016/0003-2697(84)90066-6 |
| format | Article |
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A similar error occurs with HCl hydrolysis (especially in the presence of mercaptoethanol or thioglycollic acid) if care is not taken to oxidize cysteine during reconstitution of the hydrolysate before amino acid analysis.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(84)90066-6</identifier><identifier>PMID: 6437274</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>acid hydrolysis ; amino acid analysis ; amino acid composition ; Amino Acids - analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chemical Phenomena ; Chemistry ; cysteine ; Cysteine - analysis ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation methods ; Glutathione ; Hydrochloric Acid ; Hydrogen-Ion Concentration ; Hydrolysis ; Ion Exchange ; ion-exchange analysis ; mercaptoethane sulfonic acid ; Mesna ; Peptides ; Proline - analysis ; protein structure ; Proteins</subject><ispartof>Analytical biochemistry, 1984-01, Vol.141 (2), p.429-431</ispartof><rights>1984</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-359b2e47b8ce6884d134b380125f1cdcf5e9037ec6b1bb8d694cdd72df9105ad3</citedby><cites>FETCH-LOGICAL-c417t-359b2e47b8ce6884d134b380125f1cdcf5e9037ec6b1bb8d694cdd72df9105ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(84)90066-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9144657$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6437274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gardner, Michael L.G.</creatorcontrib><title>Cysteine: A potential source of error in amino acid analysis of mercaptoethane sulfonic or hydrochloric acid hydrolysates of proteins and peptides</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Hydrolysis of proteins and peptides with mercaptoethane sulfonic acid is liable to produce overestimation of the proline content owing to the production of ninhydrin-positive material (probably cysteine) which coelutes with proline on many ion-exchange analytical systems. A similar error occurs with HCl hydrolysis (especially in the presence of mercaptoethanol or thioglycollic acid) if care is not taken to oxidize cysteine during reconstitution of the hydrolysate before amino acid analysis.</description><subject>acid hydrolysis</subject><subject>amino acid analysis</subject><subject>amino acid composition</subject><subject>Amino Acids - analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>cysteine</subject><subject>Cysteine - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>Glutathione</subject><subject>Hydrochloric Acid</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolysis</subject><subject>Ion Exchange</subject><subject>ion-exchange analysis</subject><subject>mercaptoethane sulfonic acid</subject><subject>Mesna</subject><subject>Peptides</subject><subject>Proline - analysis</subject><subject>protein structure</subject><subject>Proteins</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-v1CAUxYnRPMen30ATFsboosotlBYXJi8T_yUvcaNrQuE2g2lLBWoyX8NPLJ2ZzFJXBM7vHC4cQp4DewsM5DvGGK9qqdrXnXijGJOykg_IDpiSFeNMPSS7K_KYPEnpJ2MAopE35EYK3tat2JE_-2PK6Gd8T-_oEjLO2ZuRprBGizQMFGMMkfqZmsnPgRrrHTWzGY_Jp02fMFqz5ID5YGakaR2HMHtLi-lwdDHYwxhi2Z-Mp5NiNRlP5iWG7fJUEh1dcMneYXpKHg1mTPjsst6SH58-ft9_qe6_ff66v7uvrIA2V7xRfY2i7TuLsuuEAy563jGomwGss0ODivEWreyh7zsnlbDOtbUbFLDGOH5LXp1zyxS_VkxZTz5ZHMfyjrAm3QHn0Nb1f0HgSgLUvIDiDNoYUoo46CX6ycSjBqa3zvRWiN4K0Z3Qp860LLYXl_y1n9BdTZeSiv7yoptkzThEM1ufrpgCIWTTFuzDGcPyab89Rp2sx9mi8xFt1i74f8_xF-fTtZE</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Gardner, Michael L.G.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19840101</creationdate><title>Cysteine: A potential source of error in amino acid analysis of mercaptoethane sulfonic or hydrochloric acid hydrolysates of proteins and peptides</title><author>Gardner, Michael L.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-359b2e47b8ce6884d134b380125f1cdcf5e9037ec6b1bb8d694cdd72df9105ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>acid hydrolysis</topic><topic>amino acid analysis</topic><topic>amino acid composition</topic><topic>Amino Acids - analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>cysteine</topic><topic>Cysteine - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>Glutathione</topic><topic>Hydrochloric Acid</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydrolysis</topic><topic>Ion Exchange</topic><topic>ion-exchange analysis</topic><topic>mercaptoethane sulfonic acid</topic><topic>Mesna</topic><topic>Peptides</topic><topic>Proline - analysis</topic><topic>protein structure</topic><topic>Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gardner, Michael L.G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gardner, Michael L.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cysteine: A potential source of error in amino acid analysis of mercaptoethane sulfonic or hydrochloric acid hydrolysates of proteins and peptides</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>141</volume><issue>2</issue><spage>429</spage><epage>431</epage><pages>429-431</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>Hydrolysis of proteins and peptides with mercaptoethane sulfonic acid is liable to produce overestimation of the proline content owing to the production of ninhydrin-positive material (probably cysteine) which coelutes with proline on many ion-exchange analytical systems. A similar error occurs with HCl hydrolysis (especially in the presence of mercaptoethanol or thioglycollic acid) if care is not taken to oxidize cysteine during reconstitution of the hydrolysate before amino acid analysis.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>6437274</pmid><doi>10.1016/0003-2697(84)90066-6</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1984-01, Vol.141 (2), p.429-431 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | acid hydrolysis amino acid analysis amino acid composition Amino Acids - analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chemical Phenomena Chemistry cysteine Cysteine - analysis Fundamental and applied biological sciences. Psychology General aspects, investigation methods Glutathione Hydrochloric Acid Hydrogen-Ion Concentration Hydrolysis Ion Exchange ion-exchange analysis mercaptoethane sulfonic acid Mesna Peptides Proline - analysis protein structure Proteins |
| title | Cysteine: A potential source of error in amino acid analysis of mercaptoethane sulfonic or hydrochloric acid hydrolysates of proteins and peptides |
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