Cysteine: A potential source of error in amino acid analysis of mercaptoethane sulfonic or hydrochloric acid hydrolysates of proteins and peptides

Hydrolysis of proteins and peptides with mercaptoethane sulfonic acid is liable to produce overestimation of the proline content owing to the production of ninhydrin-positive material (probably cysteine) which coelutes with proline on many ion-exchange analytical systems. A similar error occurs with...

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Veröffentlicht in:	Analytical biochemistry 1984-01, Vol.141 (2), p.429-431
1. Verfasser:	Gardner, Michael L.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	acid hydrolysis amino acid analysis amino acid composition Amino Acids - analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chemical Phenomena Chemistry cysteine Cysteine - analysis Fundamental and applied biological sciences. Psychology General aspects, investigation methods Glutathione Hydrochloric Acid Hydrogen-Ion Concentration Hydrolysis Ion Exchange ion-exchange analysis mercaptoethane sulfonic acid Mesna Peptides Proline - analysis protein structure Proteins
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Beschreibung
Zusammenfassung:	Hydrolysis of proteins and peptides with mercaptoethane sulfonic acid is liable to produce overestimation of the proline content owing to the production of ninhydrin-positive material (probably cysteine) which coelutes with proline on many ion-exchange analytical systems. A similar error occurs with HCl hydrolysis (especially in the presence of mercaptoethanol or thioglycollic acid) if care is not taken to oxidize cysteine during reconstitution of the hydrolysate before amino acid analysis.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(84)90066-6