Identification of the herpes simplex virus type 1 gene encoding the dUTPase

Identification of the herpes simplex virus type 1 gene encoding the dUTPase

The dUTPase gene of herpes simplex virus has been identified using a novel approach. The upstream regulatory sequences, or the promoter and upstream regulatory sequences, of the immediate-early gene Vmw175 (ICP4) were inserted in front of genes mapping in the region 0.69 to 0.70 map units of the vir...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 1984-10, Vol.138 (1), p.58-68
Hauptverfasser: Preston, Valerie G., Fisher, Fiona B.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Base Sequence
Biological and medical sciences
Cell Line
Cloning, Molecular
Cricetinae
DNA Restriction Enzymes
DNA, Recombinant - metabolism
Fundamental and applied biological sciences. Psychology
Genes
Genes, Regulator
Genes, Viral
Genetics
Kidney
Kinetics
Microbiology
Plasmids
Promoter Regions, Genetic
Pyrophosphatases - genetics
Simplexvirus - enzymology
Simplexvirus - genetics
Transfection
Virology
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Zusammenfassung:The dUTPase gene of herpes simplex virus has been identified using a novel approach. The upstream regulatory sequences, or the promoter and upstream regulatory sequences, of the immediate-early gene Vmw175 (ICP4) were inserted in front of genes mapping in the region 0.69 to 0.70 map units of the virus genome to enhance transient gene expression in a transfection assay. One clone, containing a gene specifying a 1.5- kb mRNA, induced significant amounts of virus-specific dUTPase activity. The enzyme activity was abolished by insertion of a HindIII linker into the KpnI site within the coding sequences of this gene. The results show that the enzyme is virus coded and that 1.5-kb mRNA specifies the dUTPase.
ISSN:0042-6822
1096-0341
DOI:10.1016/0042-6822(84)90147-8