The Nucleotide Sequence of the Leftmost XhoI Fragment (6%) of Simian Adenovirus SA7P

Department of Medical Biochemistry, Sylvius Laboratories, University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands and 1 D.I. Ivanovsky Institute of Virology, Academy of Medical Sciences U.S.S.R., Moscow, U.S.S.R. The DNA of simian adenovirus SA7P was cloned in pBR322. The nucleotide sequences of the leftmost 2238 bp and the rightmost 188 bp of the viral genome were determined. SA7P DNA has an inverted terminal repeat of 183 bp. The sequence at the left terminus exhibits extensive homology with that of the E1 regions of human adenovirus 5, 7 and 12 DNAs. Based on this homology, the RNA coordinates and coding regions could be deduced. The sequenced SA7P DNA contains the entire E1A and part of the E1B region. Keywords: simian adenovirus, E1, inverted terminal repeat, homology Received 11 June 1984; accepted 13 July 1984.