Estriol-specific antibody for steroid assays

Estriol-specific antibody for steroid assays

Purified azobenzoyl estriol antibody IgG reacts specifically with native estriol. Fluorescence quenching of IgG by derivatized steroids provided experimentally determined binding constants over a wide range of concentrations. Since functional hydroxyl groups on the immunogen had been unmolested duri...

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Veröffentlicht in:Steroids 1971-11, Vol.18 (5), p.555-563
Hauptverfasser: Gross, S.J., Grant, J.D., Bennett, R., Wong, S-L.R., Lomax, P.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antibodies
Azo Compounds - chemical synthesis
Benzoates - chemical synthesis
Cross Reactions
Estriol - analysis
Estriol - isolation & purification
Fluorescent Antibody Technique
Immunoglobulin G
Male
Methods
Rabbits
Spectrometry, Fluorescence
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Beschreibung
Zusammenfassung:Purified azobenzoyl estriol antibody IgG reacts specifically with native estriol. Fluorescence quenching of IgG by derivatized steroids provided experimentally determined binding constants over a wide range of concentrations. Since functional hydroxyl groups on the immunogen had been unmolested during synthesis, it was possible to isolate an antibody population which did not cross-react significantly with related estratrienes.
ISSN:0039-128X
1878-5867
DOI:10.1016/0039-128X(71)90068-7