Mechanism of endonuclease banding of chromosomes
The restriction endonuclease BstNI markedly reduced the extent of Giemsa staining of the C-band regions of methanol/acetic acid-fixed mouse chromosomes air-dried onto glass slides. The enzyme reduced the amount of hybridizable satellite DNA correspondingly, indicating that its effects can be attribu...
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| Veröffentlicht in: | Experimental cell research 1984-01, Vol.155 (1), p.294-298 |
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| container_title | Experimental cell research |
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| creator | Miller, D.A. Gosden, J.R. Hastie, N.D. Evans, H.J. |
| description | The restriction endonuclease BstNI markedly reduced the extent of Giemsa staining of the C-band regions of methanol/acetic acid-fixed mouse chromosomes air-dried onto glass slides. The enzyme reduced the amount of hybridizable satellite DNA correspondingly, indicating that its effects can be attributed to cutting satellite DNA into fragments short enough to be removed from fixed chromosomes. |
| doi_str_mv | 10.1016/0014-4827(84)90793-6 |
| format | Article |
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The enzyme reduced the amount of hybridizable satellite DNA correspondingly, indicating that its effects can be attributed to cutting satellite DNA into fragments short enough to be removed from fixed chromosomes.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(84)90793-6</identifier><identifier>PMID: 6092119</identifier><identifier>CODEN: ECREAL</identifier><language>eng</language><publisher>Orlando, FL: Elsevier Inc</publisher><subject>Animals ; Azure Stains ; banding ; Biological and medical sciences ; Chromatin. Chromosome ; Chromosome Banding - methods ; chromosomes ; DNA Restriction Enzymes ; DNA, Satellite ; endodeoxyribonuclease BstNI ; Fundamental and applied biological sciences. Psychology ; hybridization analysis ; Mice ; Molecular and cellular biology ; Molecular genetics ; Nucleic Acid Hybridization</subject><ispartof>Experimental cell research, 1984-01, Vol.155 (1), p.294-298</ispartof><rights>1984</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-f4997b5905f205b9354cdecc2dc46efae6ca76bb079a73ba38529f4b6c13193</citedby><cites>FETCH-LOGICAL-c417t-f4997b5905f205b9354cdecc2dc46efae6ca76bb079a73ba38529f4b6c13193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4827(84)90793-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9114913$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6092119$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miller, D.A.</creatorcontrib><creatorcontrib>Gosden, J.R.</creatorcontrib><creatorcontrib>Hastie, N.D.</creatorcontrib><creatorcontrib>Evans, H.J.</creatorcontrib><title>Mechanism of endonuclease banding of chromosomes</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>The restriction endonuclease BstNI markedly reduced the extent of Giemsa staining of the C-band regions of methanol/acetic acid-fixed mouse chromosomes air-dried onto glass slides. The enzyme reduced the amount of hybridizable satellite DNA correspondingly, indicating that its effects can be attributed to cutting satellite DNA into fragments short enough to be removed from fixed chromosomes.</description><subject>Animals</subject><subject>Azure Stains</subject><subject>banding</subject><subject>Biological and medical sciences</subject><subject>Chromatin. Chromosome</subject><subject>Chromosome Banding - methods</subject><subject>chromosomes</subject><subject>DNA Restriction Enzymes</subject><subject>DNA, Satellite</subject><subject>endodeoxyribonuclease BstNI</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hybridization analysis</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Nucleic Acid Hybridization</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFO3DAQhi1UBAvlDUDaQ4XgEDrjOI59QUKoLUggDu3dciaTrqsk3tq7SLx9s-xqj-U00vzfjH59Qpwj3CCg_gqAqlBG1ldGXVuobVnoAzFDsFBIJeUnMdsjx-Ik5z8AYAzqI3GkwUpEOxPwzLTwY8jDPHZzHts4rqlnn3ne-LEN4-_NnhYpDjHHgfNncdj5PvPZbp6Kn9-__bp_KJ5efjze3z0VpLBeFZ2ytm4qC1UnoWpsWSlqmUi2pDR3njX5WjfNVNvXZeNLU0nbqUYTlmjLU3G5_bpM8e-a88oNIRP3vR85rrMzKI0BVX0IoioBZWUmUG1BSjHnxJ1bpjD49OYQ3Man28hyG1nOKPfu0-np7GL3f90M3O6PdgKn_Msu95l83yU_Ush7zCIqi-WE3W4xnpS9Bk4uU-CRuA2JaeXaGP7f4x_xro_t</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Miller, D.A.</creator><creator>Gosden, J.R.</creator><creator>Hastie, N.D.</creator><creator>Evans, H.J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19840101</creationdate><title>Mechanism of endonuclease banding of chromosomes</title><author>Miller, D.A. ; Gosden, J.R. ; Hastie, N.D. ; Evans, H.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-f4997b5905f205b9354cdecc2dc46efae6ca76bb079a73ba38529f4b6c13193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Azure Stains</topic><topic>banding</topic><topic>Biological and medical sciences</topic><topic>Chromatin. Chromosome</topic><topic>Chromosome Banding - methods</topic><topic>chromosomes</topic><topic>DNA Restriction Enzymes</topic><topic>DNA, Satellite</topic><topic>endodeoxyribonuclease BstNI</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hybridization analysis</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Nucleic Acid Hybridization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miller, D.A.</creatorcontrib><creatorcontrib>Gosden, J.R.</creatorcontrib><creatorcontrib>Hastie, N.D.</creatorcontrib><creatorcontrib>Evans, H.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miller, D.A.</au><au>Gosden, J.R.</au><au>Hastie, N.D.</au><au>Evans, H.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism of endonuclease banding of chromosomes</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>155</volume><issue>1</issue><spage>294</spage><epage>298</epage><pages>294-298</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>The restriction endonuclease BstNI markedly reduced the extent of Giemsa staining of the C-band regions of methanol/acetic acid-fixed mouse chromosomes air-dried onto glass slides. The enzyme reduced the amount of hybridizable satellite DNA correspondingly, indicating that its effects can be attributed to cutting satellite DNA into fragments short enough to be removed from fixed chromosomes.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>6092119</pmid><doi>10.1016/0014-4827(84)90793-6</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Experimental cell research, 1984-01, Vol.155 (1), p.294-298 |
| issn | 0014-4827 1090-2422 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Azure Stains banding Biological and medical sciences Chromatin. Chromosome Chromosome Banding - methods chromosomes DNA Restriction Enzymes DNA, Satellite endodeoxyribonuclease BstNI Fundamental and applied biological sciences. Psychology hybridization analysis Mice Molecular and cellular biology Molecular genetics Nucleic Acid Hybridization |
| title | Mechanism of endonuclease banding of chromosomes |
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