Mechanism of endonuclease banding of chromosomes

Mechanism of endonuclease banding of chromosomes

The restriction endonuclease BstNI markedly reduced the extent of Giemsa staining of the C-band regions of methanol/acetic acid-fixed mouse chromosomes air-dried onto glass slides. The enzyme reduced the amount of hybridizable satellite DNA correspondingly, indicating that its effects can be attribu...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Experimental cell research 1984-01, Vol.155 (1), p.294-298
Hauptverfasser: Miller, D.A., Gosden, J.R., Hastie, N.D., Evans, H.J.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Azure Stains
banding
Biological and medical sciences
Chromatin. Chromosome
Chromosome Banding - methods
chromosomes
DNA Restriction Enzymes
DNA, Satellite
endodeoxyribonuclease BstNI
Fundamental and applied biological sciences. Psychology
hybridization analysis
Mice
Molecular and cellular biology
Molecular genetics
Nucleic Acid Hybridization
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The restriction endonuclease BstNI markedly reduced the extent of Giemsa staining of the C-band regions of methanol/acetic acid-fixed mouse chromosomes air-dried onto glass slides. The enzyme reduced the amount of hybridizable satellite DNA correspondingly, indicating that its effects can be attributed to cutting satellite DNA into fragments short enough to be removed from fixed chromosomes.
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(84)90793-6