New fluorescence of nonenzymatically glucosylated human serum albumin

Glucosylated human serum albumin (G-HSA) obtained under incubation with glucose at 37° C for 8 days showed a new fluorescence with a maximum at 430 nm, resulting in quenching of the fluorescence of only one tryptophan residue on HSA. The quantum yield of new fluorescence is 0.024 at 25° C. The analy...

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Veröffentlicht in:FEBS letters 1984-10, Vol.176 (1), p.27-31
Hauptverfasser: Sakurai, Tamiko, Takahashi, Hiroshi, Tsuchiya, Seishi
Format: Artikel
Sprache:eng
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Zusammenfassung:Glucosylated human serum albumin (G-HSA) obtained under incubation with glucose at 37° C for 8 days showed a new fluorescence with a maximum at 430 nm, resulting in quenching of the fluorescence of only one tryptophan residue on HSA. The quantum yield of new fluorescence is 0.024 at 25° C. The analysis of the excitation spectra allowed us to conclude the absence of energy transfer. In G-HSA, non-disulfide cross-linking hexamer was confirmed by SDS-PAGE.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)80905-9